Trace level enrichment and clean-up of β-lactam antibiotic residues in edible chicken tissues and feathers by solid phase extraction for quantitative determination utilizing liquid chromatography

ABSTRACT. A simple analytical method for the extraction and determination of three β-lactam antibiotics in chicken tissues feather samples was developed validated using solid phase (SPE) liquid chromatography-ultraviolet, LC-UV, detection. The analytes were extracted with strong anion exchange (SAX) cartridges extract clean-up enrichment before introduction into LC system. validation carried out based on recommendations from Commission Decision 2002/657/EC. Under optimised conditions, proposed offers correlation coefficients r2 ≥ 0.9991 all studied both matrices. Percent recovery results found to vary between 79.7-101.8% 94.2-101.6% feathers tissues, respectively, relative standard deviation, RSD < 8.4%. limits detection (LOD) range 0.1-0.13 µg kg-1 0.1-0.15 while quantification (LOQ) 0.3-0.38 0.3-4.5 respectively. good sensitivity selectivity demonstrating that would be widely used other trace level residues having similar physical chemical properties matrices varying origins.
 
 KEY WORDS: Chicken feathers, β-Lactams, Solid extraction, LC-UV
 Bull. Chem. Soc. Ethiop. 2022, 36(3), 503-519. DOI: https://dx.doi.org/10.4314/bcse.v36i3.2